Pipetas graduadas | 5 | Material de laboratorio
What Are Graduated Pipettes and How to Use Them?
Introduction to Graduated Pipettes
- The video introduces graduated pipettes, which are narrow tubes typically made of glass with a conical shape at the bottom and a narrower section at the top.
- These pipettes are calibrated for measuring precise volumes, available in various sizes, allowing for accurate measurements such as 15.6 milliliters.
Usage of Graduated Pipettes
- To use a graduated pipette correctly, one must suck liquid using an appropriate pipette filler until it slightly exceeds the zero mark.
- After that, carefully release some liquid until it reaches the desired volume mark; this step requires precision as once poured out, you cannot retrieve the liquid.
Important Considerations
- When using inverted graduated pipettes, it's crucial not to expel the last drop since they are calibrated to account for that final volume.
- A fundamental safety rule is never to suck liquid into the pipette using your mouth; always use a proper pipette filler. The speaker emphasizes this by comparing new and worn-out rubber bulbs.
Conclusion