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Tutorial cara menggunakan photometer 4010 ( analis kesehatan )
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Tutorial cara menggunakan photometer 4010 ( analis kesehatan )

Procedure for Operating the Equipment

Initial Setup

  • The procedure begins with turning on the equipment, which emits a sound due to its wavelength settings. The specific wavelength being set is 546 nm.
  • Adjustments are made to the program settings, including concentration factors that need to be inputted correctly.

Sample Preparation

  • Before starting measurements, it is essential to rinse the equipment and prepare samples properly. A minimum sample volume of 1 milliliter is required for accurate readings.
  • The blank sample must be zeroed out to ensure that only the sample's concentration is measured, eliminating any interference from other solutions.

Mixing and Measurement

  • Proper mixing of samples is emphasized; inadequate mixing can lead to inaccurate results.
  • Hemoglobin (Hb) levels are discussed in relation to oxygen binding; incorrect handling can lead to significant errors in measurement.

Sample Analysis Process

Observations During Testing

  • It’s noted that if samples are left too long without proper mixing, sedimentation occurs, affecting accuracy. Cells settle at the bottom and require re-mixing before analysis.
  • Multiple samples can be tested simultaneously; however, care must be taken regarding how many blanks are used for calibration.

Final Steps

  • Each participant should ensure they have sufficient blank samples prepared before proceeding with their tests.
Video description

Fotometer ialah alat untuk menangkap kekuatan cahaya atau interaksi cahaya yang ditransmisikan atau pengukuran berdasarkan cahaya dengan sumber radiasi elektromagnetik. Nama lain dari fotometer yang dipraktikumkan ialah Hospitex Diagnostic artinya instrument yang biasa digunakan pada rumah sakit yang menggunakan sample klinis misalnya serum darah. Komponen-komponen fotometer hampir sama dengan spektrofotometer meliputi sumber cahaya atau sumber radiasi yaitu lampu halogen, kemudian filter, tempat sample atau kuvet, detector ialah silicon, dan sample klinis yaitu serum darah. Prinsip kerja fotometer yaitu sampel yang telah diinkubasi kemudian disedotkan pada aspirator sehingga masuk ke dalam kuvet dan dibaca oleh sinar cahaya kemudian sampel akan disedot kembali dengan pompa peristaltik menuju ke pembuangan. Sampel yang digunakan harus dimasukkan dalam inkubator. Hal ini agar reagen-reagen dalam sampel bekerja secara maksimal.