Antibiograma Método Kirby Bauer

Antibiogram Procedure Using Kirby-Bauer Method

Overview of the Antibiogram Process

• The antibiogram is performed after isolating the problematic bacteria in pure culture, utilizing a sterilized inoculating loop to create a suspension.

• A saline solution with turbidity equivalent to 0.5 on the McFarland scale is prepared for inoculation, ensuring accurate bacterial density for testing.

• Sterilized tweezers are used to place antibiotic discs on the agar plate, maintaining a distance of at least 15 mm from the edge and sufficient spacing between discs to avoid overlapping inhibition zones.

Incubation and Interpretation

• After placing the discs, plates should rest at room temperature for about 15 minutes before incubation at 37°C for 24 hours.