HPLC Instrumentation 19 - Autosampler Sampling Valve

Introduction to Sample Introduction in HPLC

This section introduces the challenge of introducing samples into a high-pressure flow path in High-Performance Liquid Chromatography (HPLC). The technique used in HPLC involves parking the sample in a sample loop at ambient pressure before transferring it onto the column.

Sample Introduction Technique in HPLC

• In HPLC, the technique used for sample introduction is to first park the sample in a sample loop at ambient pressure.

• After parking the sample, the flow is redirected through a valve and the sample is transferred out of the loop and onto the column.

Six Port Valve in Manual Injector

• A typical six port valve contained in a manual injector is shown.

• The sample is introduced into the sample loop using a manual syringe.

• To vary the injection volume, the entire sample loop would have to be physically changed as it needs to be completely filled for each injection.

• The transcript provided does not specify any language other than English, so all notes are written in English.

• The timestamps provided are associated with their respective bullet points.