Técnicas básicas de Microbiología: Siembra en cultivo sólido y extensión con asa de Drigalsky

How to Transfer Liquid Culture to Solid Medium

Introduction to the Procedure

• The video demonstrates the process of transferring a liquid culture into a solid medium using an instrument called Asa di Gransky, which is a triangular-ended glass rod designed for even spreading of the culture.

Preparing the Culture

• A pipette is used to take 100 microliters of the sterile liquid culture. This amount is then deposited in the center of a sterile plate.

• After discarding the pipette tip, the Asa di Gransky is sterilized by passing it through alcohol and then through a flame, ensuring that it is free from contaminants before use.

Extending the Culture